Device

Part:BBa_K5090009:Design

Designed by: Manuel Marques Vilar   Group: iGEM24_Stony-Brook   (2024-10-01)


GFP Device Regulated By lacO and Kink Turn


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Many variants of GFP have been developed which included sfGFP, EGFP, and cGFP to tailor and fine tune for various purposes. Wild-Type GFP is the dimmest version out of them all, and could potentially be useful in assays that need to avoid phototoxicity or interference with other experiments.



Source

This part is a functional composite part including a an Anderson promoter (BBa_J23100), two lac operators (BBa_K079017), a kink turn (BBa_K4140015), an Anderson RBS (BBa_J61100), GFP (BBa_K5090003), and a T1 terminator (BBa_B0010).

References